Onion cell lab report pdf

Unlike animal cells such as cheek cells the cell wall of an onion and other plants are made up of cellulose, which protects the cell and maintains its shape. Skin cells each person will make their own slide 1. Peel off a leaf from half a piece of onion and using the forceps, pull out a piece of transparent onion peel epidermis from the leaf. List 4 similarities all cells possess no matter what kingdom. The cheek epithelium cell is the only one that has centrioles, the barrelshaped organelle that is responsible. Furthermore, the onion cells are bricklike in shape while the human cheek cells are rounded. Create a lab report beginning on page 70 of your inb. Osmosis is the diffusion of water from an area of low concentration to an area of high concentration across a semipermeable membrane. The vacuole is prominent and present at the centre of the cell. The main difference between onion cell and human cheek cell is that the onion cell is a plant cell with a cell wall made up of cellulose whereas the human cheek cell is an animal cell without a cell wall. To find out what an animal cells look like under a microscope. Eukaryotic cell lab report essay example graduateway.

Students will use a microscope to examine the structure of the onion epidermal cell with particular emphasis on the cell wall, cell membrane, cytoplasm and nucleus. Onion cell plasmolysis lab version 1 free download as word doc. To observe and identify major animal cell structures and to relate the structure of the cell to its function. The steps used to remove and expose dna from its cell. The inner surface of each layer has a thin layer of tissue thats easy. Introduction both animals and plants are eukaryotic cells because it contains membranebound organelles.

The purpose of this lab is to compare the three different types of solutions affect on the relative size of the vacuole to the cell, the. Written in 3rd person no i, my, we or our materials. Describe what happens at each step, and why it acts to separate the parts of the cell. This makes a root tip an excellent tissue to study the stages of cell division. Use complete sentences and paragraphs for your answers on the back of this. Better understand the process and stages of mitosis.

Plants cell lab write up friends of the environment. Youssef gharib brief description of osmosis in red onion cells. Make a drawing of one onion cell, labeling all of its parts as you observe them. Lab report on the extraction of onion dna free essays. The difference between plant cell and an animal cell however is that plant cell have cell wall, chloroplast and a permanent vacuole. Today%for%this%laboratory%we%will%be%using%a%compound%light%microscope. Draw and label the onion cell at both low and high power. Then, another onion root tip was prepared and area z was located. As in all plant cells, the cell of an onion peel consists of a cell wall, cell membrane, cytoplasm, nucleus and a large vacuole. Onion tissue provides excellent cells to study under the microscope. To see if each phase of mitosis happens for an equal amount of time in onion root tip cells.

Students compare plant and animal cells by observing cells from an onion as well as cells from their own cheek. In addition, it is the only cell that has a chloroplast, where the photosynthesis can happen. Examining onion tissue lab a characteristic that distinguishes plant cells from animal cells is the cell wall. There will be no net loss or gain of water into or out of the cell. The onion epidermis cell is the only cell that has a cell wall. This presentation walks students stepbystep through the procedures and analysis for a cheek and onion cell lab. Dna extraction of a musa acuminata biology lab report \analyze and interpret a number of steps are required to isolate dna from cellular contents. Onion roots the bracketed area of this diagram shows where you are most likely to find dividing cells. Student laboratory onion cell staining and cell size. Be sure that the person reading your answer will know what the. The heat treatment softens the phospholipid in the cell membrane. Cell biology preparing wet mount lab page 1 preparing wet mount lab materials onion slice microscope microscope slides water cover slips eyedropper iodine methylene blue tweezers worksheet procedure preparing the wet mount slide of onion skin 1.

Careful, iodine is not toxic, but it will stain both yo ur skin and your clothes. Obtain a sheet of lens tissue paper with lens cleaning solution and clean all the lenses of your microscope gently. First, with a prepared slide, area x and y were located and each counted and recorded of what stages were observed. Problem, hypothesis and prediction in this lab experiment, hannah was to analyze an onion root tip under a microscope to observe the mitotic stages that occur in the cells of onion root tips. Extraction of dna from onion university of wisconsin. The objective of this experiment was to calculate the percentage of cells in each of the phases of mitosis. In a cell, dna is located abundantly in the form of chromatin in the nucleolus of a cell britannica, 2018.

Start with the low power objective and work your way until you have focused the onion cell using the medium power objective. Always hold the slide by the edges or you will leave fingerprints on the slide. The cells of an onion skin are generally rectangular in shape and range in size from. Materials onion, toothpick, safranin solution, iodine solution, distilled water, dropper, forceps, cover slip, glass slide, filter paper, compound microscope, gloves methods onion cells 1 a glass slide is taken and some distilled water was poured onto it. Preparing onion cell slides is a convenient way to observe simple plant cells under the light microscope. The cell cycle results in the formation of two genetically identical daughter cells from the division of a parent cell. Mitosis and chromosome number lab report bio 101 studocu. This video describes how to observe plasmolysis cytoplasm retracting from the cell wall when the cell looses water in onion epidermal cells. Interphase consist of g 1 growth phase or gap, s synthesis phase and g 2 second growth phase or gap 2 then followed by mitosis. Lab report includes abstract, introduction, methods, result and discussion. Pdf studies on onion cells allium cepa with a foldscope. Wongs 7th period biohonors class, we did a lab experiment on the processes of mitosis and the different phases as seen under a microscope.

To create a wetmount slide and use a drawtrough staining technique to examine cell structures to calculate the field diameter of a microscope and calculate average onion epidermis cell size prelab. The cellular organelles that were observed were a cell wall, nucleus, and cytoplasm. Because each cell divides independently of the others, a root tip contains cells at different stages of the cell cycle. Looking through the microscope and comparing the onion cell to the whitefish cell helped us gain an understanding of the difference between them.

If i look at my cheek cell scraping under a microscope and put iodine on it, then i will see the different parts of the cell including the nucleus, cytoplasm, and cell membrane. Plants have levels of organization for structure and function, including cells, tissues, organs, organ systems, and the whole organism. Name 2 things cell membranes allow into the cell so it can continue to live. The present video focussess on the procedure,observations of the experimentmitotic cell division in onion root tip. Onion cell lab y9 staining cell biology free 30day trial. An onion is made up of layers that are separated by a thin membrane. When you see these cells in microscope you can clearly find one difference that onion cell has cell wall while cheek cell does not have. Taking it in turns finely dice your onion piece with the razor blade and transfer to the tube with the pestle. Homogenization, deproteinization, and precipitation. Put the epidermis in the watch glass containing distilled water. Crush and grind the onion pieces until a homogenous, smooth paste is a chieved. The main cell structures are easy to see when viewed with the microscope at medium power. The onion cell was also distinguished as a plant cell due to its rectangular shape. Prepare an onion slide by taking a small piece of onion and making a wet mount.

In g 1 volume of cytoplasm increase and the protein start to synthesis. Place this slide on the stage and under the stage clips. Onion root tip lab report portfolio of hannah scott. Onion cells under the microscope requirements, preparation and. The purpose of this lab is to examine a plant cells response to a change in the cells water environment.

Onion epidermis as a new model to study the control of. Difference between onion cell and human cheek cell. In this lab, you will prepare and observe cells from both a plant onion and an animal your own cheek. Lab materials whole white onion soaked in water overnight microscopes 23 students per scope microscope slides water bottle cover slips eyedropper pipette iodine tweezers worksheet procedure preparing the wet mount slide of onion skin 1. Plasmolysis in onion epidermal cells biology lab techniques. Onion cells under the microscope requirements, preparation and observation. Add a small drop of iodine stain directly to the tissue. It was stained with neutral red it accumulates in the cytoplasm of the cell. The common features of animal cell and plant cell are nucleus, cytoplasm, cell membrane, mitochondria and ribosomes. Microscope prepared slides of a longitudinal section of allium onion. Onion cell is a plant cell and cheek cell is an animal cell. For this microscope experiment, the thin membrane will be used to observe the cells. Students make a wetmount slide of their own cheek cells and another of onion cells. Humans have 46 chromosomes in each of our cells while the onion cell has about.

While photosynthesis takes place in the leaves of an onion containing chloroplast, the little glucose that is produced from this process is converted in to starch starch granules and stored in the bulb. Observing onion cells under a microscope blog, she wrote. Observe the onion cell under both low and high power. Increase the number of organelles such as mitochondria, endoplasmic reticulum and golgi apparatus. Carefully cut away a small, single layered piece of onion 12 cm wide. So you have to differentiate between a plant cell and an animal cell. To extract the dna, the nucleus has to be released from the onion cell which. The nucleus is present at the periphery of the cytoplasm. The homogenization step involves the heating and blending of the onion tissue in order to break down the cells.

Onion cells, microscope skills and worksheet teaching. In this lab you will be observing plant cells onion in the various stages of mitosis, and make time calculations based on the data you collect. Nucleus, cell wall, cytoplasm, and the nucleolus within the cells of an onion and a cheek. Prepare your own specimens of onion root in which you can visualize all of the stages of mitosis. The objectives of this lab exercise are for you to. The bulb of an onion is formed from modified leaves. This lab was an experiment designed to analyze how many cells could be observed in each part of mitosis for different areas of an onion root. In a growing plant root, the cells at the tip of the root are constantly dividing to allow the root to grow. To prepare stained temporary mount of onion peel materials required. At minimum you should observe the nucleus, cell wall, and cytoplasm. A greenhouse experiment was carried out comparing the growth of various plant species in nonfumigated, fumigated, and fumigatedinoculated.

For this experiment, the thin membrane will be used to observe the onion cells. Download as doc, pdf, txt or read online from scribd. Red onion, microscope, microscope slide, cover slip, 15% sodium chloride solution, bottle of distilled water. Other diffrences will not be clear with ordinary microscope. In this lab students will be able to observe the following organelles. Most of the cells are spent in the interphase stages. Get a glass slide and place a small amount of onion tissue in the center.

Onion, knife, compound microscope, glass slide, cover slip, and iodine used for staining. Introductionafter reading through the lab procedures in parts a and b, write a thorough introductionexplaining the. An onion is a multicellular consisting of many cells plant organism. Objective to develop stained temporary mounts of onion peel and cheek cells and analyse the difference between the plant cells and the animal cells. To elucidate the role of cellulose microfibrils in the control of growth anisotropy, a link between their net orientation, in vitro cell wall extensibility, and anisotropic cell expansion was studied during development of the adaxial epidermis of onion allium cepa bulb scales using polarization confocal microscopy, creep tests, and light microscopy. Onion is used because of its abundance and low starch content. Mitosis in onion root tip cells objective after completing this exercise, you should be able to.